Phylogenetic relationships had been identical in Bayesian inference, maximum likelihood and maximum parsimony estimates (?lnL = 1399.28612; parsimony size = 141). Support values listed above nodes are Bayesian probabilities according to 5,000,000 resamples, maximum likelihood bootstrap help values considering 1,000 bootstrap replicates, and maximum parsimony bootstrap help values predicated on 2,000 bootstrap replicates. Help values are shown just for clades of Sotalia guianensis and Sotalia fluviatilis haplotypes, and also for the monophyly of Sotalia. Branch lengths are proportional to likelihood that is maximum divergence; scale suggests portion of series divergence. Haplotypes 1–14, highlighted in bold, had been present in our correspond and study to GenBank accession #EU022531–EU022544.

All eyeball?derived sequences decided by BLAST to be of cetacean origin provided species?specific character that is autapomorphic indicative of molecular positional homologies with Sotalia guianensis with no other types, unambiguously assigning all eyeballs of cetacean origin to Sotalia guianensis. The control area and Cytochrome b information sets have two and six diagnostic web web sites, correspondingly, that assign all eyeball that is cetacean to Sotalia guianensis and unambiguously distinguish it from Sotalia fluviatilis and Inia spp. ( dining Table 1; online Appendix S1, S2).

Control area | BEL | MAO | PVH | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|

Variable position | 39 | 136 | 139 | 160 | 175 | 231 | 256 | 269 | 270 | 362 | 381 | 387 | 404 | 463 | 504 | 559 | Haplotype occurence | ||

Inia spp. | A | T | the | G | C | G | G/A | A | A | T | C | C/T | C/T | C | C/T | A | ? | ? | ? |

Sotalia fluviatilis | T | T | A | G | C/T | G | C/T | T | C | C/T | C | T | T | C | T | A | ? | ? | ? |

Sotalia guianensis | C | G/T | A/C | A | C/T | G/A | C/T | C/T | C/T | C/T | C/G | C/T | C/T | C/T | C/T | C/A | 22 | 11 | 1 |

CR_Hap01 | C | T | A | A | T | A | C | C | C | C | C | T | C | C | T | the | 1 | ? | ? |

CR_Hap02 | C | T | A | A | T | A | C | T | C | C | C | C | T | C | T | A | 2 | ? | ? |

CR_Hap03 | C | T | A | A | T | A | C | C | C | C | C | C | T | C | T | the | 6 | 2 | ? |

CR_Hap04 | C | T | A | A | T | A | C | T | C | C | C | T | C | C | T | A | 3 | 5 | 1 |

CR_Hap05 | C | T | A | A | T | A | C | T | C | T | C | T | C | C | T | the | 1 | 2 | ? |

CR_Hap06 | C | T | the | A | C | the | T | T | T | C | C | T | T | C | T | the | 1 | ? | ? |

CR_Hap07 | C | T | A | A | T | A | C | T | C | C | C | C | C | C | T | A | 3 | ? | ? |

CR_Hap08 | C | G | C | A | T | G | C | T | C | C | C | T | C | C | T | the | 1 | ? | ? |

CR_Hap09 | C | T | A | A | T | G | C | T | C | C | C | T | C | C | T | the | 1 | ? | ? |

CR_Hap10 | C | T | A | A | T | A | C | C | C | C | C | T | T | C | T | the | 1 | ? | ? |

CR_Hap11 | C | T | A | A | T | A | C | C | C | C | C | C | T | T | T | A | ? | 1 | ? |

CR_Hap12 | C | T | A | A | T | A | C | C | C | C | C | C | T | C | T | C | ? | 1 | ? |

CR_Hap13 | C | T | the | A | C | the | C | T | T | C | C | T | T | C | C | the | 1 | ? | ? |

CR_Hap14 | C | T | A | A | T | A | C | C | C | C | G | C | T | C | T | the | 1 | ? | ? |

Cytochrome b | |||||||||||||||||||

Variable position | 49 | 78 | 124 | 297 | 367 | 396 | 462 | 501 | 528 | 531 | 561 | ||||||||

Inia spp. | G | T | A | C | G | C | T | T | A | C | T | ? | ? | ? | |||||

Sotalia fluviatilis | G | C | A | G | G | C | T | the | A | C | T | ? | ? | ? | |||||

Sotalia guianensis | G/A | C/T | G/A | A | G/A | C/T | C | G | C | T | C | 16 | 7 | 1 | |||||

CB_Hap01 | G | C | A | A | G | C | C | G | C | T | C | 8 | 4 | 1 | |||||

CB_Hap02 | A | C | A | the | G | C | C | G | C | T | C | 1 | ? | ? | |||||

CB_Hap03 | G | T | A | A | G | C | C | G | C | T | C | 1 | ? | ? | |||||

CB_Hap04 | G | C | G | A | G | T | C | G | C | T | C | 3 | 1 | ? | |||||

CB_Hap05 | G | C | G | A | A | T | C | G | C | T | C | 3 | 2 | ? |

- Web web web Sites highlighted in bold unambiguously assign the eyeball that is unknown to Sotalia guianensis. Those perhaps perhaps not highlighted differentiate noticed haplotypes. Control area haplotypes 1–14 match GenBank accession numbers EU022531?EU022544, while cytochrome b haplotypes 1–5 match GenBank accession numbers EU022545?EU022549. BEL = Belem, MAO = Manaus, PVH = Porto Velho.